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human peripheral blood mononuclear cell isolation medium  (Beijing Solarbio Science)


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    Beijing Solarbio Science human peripheral blood mononuclear cell isolation medium
    Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse <t>PBMCs</t> ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues
    Human Peripheral Blood Mononuclear Cell Isolation Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+blood+mononuclear+cell+isolation+medium/Medium/pmc13014779-47-11-19
    Average 99 stars, based on 10844 article reviews
    human peripheral blood mononuclear cell isolation medium - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2"

    Article Title: The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-026-02740-3

    Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse PBMCs ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues
    Figure Legend Snippet: Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse PBMCs ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues

    Techniques Used: Metabolomic, Injection, Expressing, Immunohistochemical staining

    Related Articles

    Cell Isolation:

    Article Title: The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2
    Article Snippet: .. Peripheral mononuclear macrophages (PBMCs) were separated from blood samples by using human peripheral blood mononuclear cell isolation medium (P9010, Solarbio) or mouse peripheral blood mononuclear cell isolation medium (P6340, Solarbio). .. Primary mouse bone marrow-derived macrophages (BMDMs) from the bone marrow of wild-type (WT) and indicated knockout mice were grown for 6 days in RPMI1640 containing macrophage colony-stimulating factor (10 ng/ml; #100–21-10, PeproTech), 10% fetal bovine serum (FBS), and 1% penicillin–streptomycin antibiotics (NCM Biotech, C100C5).

    Article Title: The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2.
    Article Snippet: .. Peripheral mononuclear macrophages (PBMCs) were separated from blood samples by using human peripheral blood mononuclear cell isolation medium (P9010, Solarbio) or mouse AR TIC LE IN PR ES S peripheral blood mononuclear cell isolation medium (P6340, Solarbio). .. Primary mouse bone marrow-derived macrophages (BMDMs) from the bone marrow of wild-type (WT) and indicated knockout mice were grown for 6 days in RPMI1640 containing macrophage colonystimulating factor (10 ng/ml; #100-21-10, PeproTech), 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin antibiotics (NCM Biotech, C100C5).



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    Beijing Solarbio Science human peripheral blood mononuclear cell isolation medium
    Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse <t>PBMCs</t> ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues
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    Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse <t>PBMCs</t> ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues
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    Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse PBMCs ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues

    Journal: Cell Communication and Signaling : CCS

    Article Title: The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2

    doi: 10.1186/s12964-026-02740-3

    Figure Lengend Snippet: Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse PBMCs ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues

    Article Snippet: Peripheral mononuclear macrophages (PBMCs) were separated from blood samples by using human peripheral blood mononuclear cell isolation medium (P9010, Solarbio) or mouse peripheral blood mononuclear cell isolation medium (P6340, Solarbio).

    Techniques: Metabolomic, Injection, Expressing, Immunohistochemical staining